Derivative Assay:Article Title: Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions.
Article Snippet: .. CHO‐K1 (ATCC CCL‐61), CHO‐DP12 cell line (ATCC CRL‐ 12445 clone #1934) which produces an anti‐IL8‐IgG, and CHO‐ SK15‐EPO (an in‐house CHO‐K1 (ATCC CCL‐61) derived cell line, expressing recombinant human erythropoietin (EPO) in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al. 2019) were grown at 37°C under 5% CO2 and 80% humidity at 170 rpm in BalanCD CHO Growth A (FujiFilm, 91128) cell culture medium supplemented with 4 mM L‐glutamine (Sigma Aldrich, G7513) in a Climo‐Shaker (Kühner). .. Cells were cultured in a working volume of 5 mL of cell culture media in 50mL TPP® TubeSpin BioReactor Tubes (Merck, Z761028‐180EA), with routine passaging at 2 × 105 cells/mL.
Article Title: Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions
Article Snippet: .. CHO‐K1 (ATCC CCL‐61), CHO‐DP12 cell line (ATCC CRL‐12445 clone #1934) which produces an anti‐IL8‐IgG, and CHO‐SK15‐EPO (an in‐house CHO‐K1 (ATCC CCL‐61) derived cell line, expressing recombinant human erythropoietin (EPO) in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al. ) were grown at 37°C under 5% CO 2 and 80% humidity at 170 rpm in BalanCD CHO Growth A (FujiFilm, 91128) cell culture medium supplemented with 4 mM l ‐glutamine (Sigma Aldrich, G7513) in a Climo‐Shaker (Kühner). .. Cells were cultured in a working volume of 5 mL of cell culture media in 50 mL TPP® TubeSpin BioReactor Tubes (Merck, Z761028‐180EA), with routine passaging at 2 × 10 5 cells/mL.
Article Title: Label-free quantitative proteomics analysis of producer and non-producer Chinese Hamsters Ovary (CHO) cells under ER stress conditions
Article Snippet: .. CHO-K1 (non-producer, ATCC CCL-61), CHO DP-12 (a recombinant human anti-IL-8 producer, ATCC CRL-12445 clone #1934) and CHO SK15-EPO (an in-house CHO-K1 derived cell line, expressing recombinant human erythropoietin in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al., 2019) were grown in BalanCD CHO Growth A (FujiFilm, NL) supplemented with 4 mM L-glutamine (Sigma Aldrich, IE) and maintained in a ClimoShaker ISF1-X (Kühner, CH) at 37 ◦C under 5% CO2 and 80% humidity with constant shaking (170 RPM). ..
Expressing:Article Title: Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions.
Article Snippet: .. CHO‐K1 (ATCC CCL‐61), CHO‐DP12 cell line (ATCC CRL‐ 12445 clone #1934) which produces an anti‐IL8‐IgG, and CHO‐ SK15‐EPO (an in‐house CHO‐K1 (ATCC CCL‐61) derived cell line, expressing recombinant human erythropoietin (EPO) in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al. 2019) were grown at 37°C under 5% CO2 and 80% humidity at 170 rpm in BalanCD CHO Growth A (FujiFilm, 91128) cell culture medium supplemented with 4 mM L‐glutamine (Sigma Aldrich, G7513) in a Climo‐Shaker (Kühner). .. Cells were cultured in a working volume of 5 mL of cell culture media in 50mL TPP® TubeSpin BioReactor Tubes (Merck, Z761028‐180EA), with routine passaging at 2 × 105 cells/mL.
Article Title: Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions
Article Snippet: .. CHO‐K1 (ATCC CCL‐61), CHO‐DP12 cell line (ATCC CRL‐12445 clone #1934) which produces an anti‐IL8‐IgG, and CHO‐SK15‐EPO (an in‐house CHO‐K1 (ATCC CCL‐61) derived cell line, expressing recombinant human erythropoietin (EPO) in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al. ) were grown at 37°C under 5% CO 2 and 80% humidity at 170 rpm in BalanCD CHO Growth A (FujiFilm, 91128) cell culture medium supplemented with 4 mM l ‐glutamine (Sigma Aldrich, G7513) in a Climo‐Shaker (Kühner). .. Cells were cultured in a working volume of 5 mL of cell culture media in 50 mL TPP® TubeSpin BioReactor Tubes (Merck, Z761028‐180EA), with routine passaging at 2 × 10 5 cells/mL.
Article Title: Label-free quantitative proteomics analysis of producer and non-producer Chinese Hamsters Ovary (CHO) cells under ER stress conditions
Article Snippet: .. CHO-K1 (non-producer, ATCC CCL-61), CHO DP-12 (a recombinant human anti-IL-8 producer, ATCC CRL-12445 clone #1934) and CHO SK15-EPO (an in-house CHO-K1 derived cell line, expressing recombinant human erythropoietin in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al., 2019) were grown in BalanCD CHO Growth A (FujiFilm, NL) supplemented with 4 mM L-glutamine (Sigma Aldrich, IE) and maintained in a ClimoShaker ISF1-X (Kühner, CH) at 37 ◦C under 5% CO2 and 80% humidity with constant shaking (170 RPM). ..
Recombinant:Article Title: Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions.
Article Snippet: .. CHO‐K1 (ATCC CCL‐61), CHO‐DP12 cell line (ATCC CRL‐ 12445 clone #1934) which produces an anti‐IL8‐IgG, and CHO‐ SK15‐EPO (an in‐house CHO‐K1 (ATCC CCL‐61) derived cell line, expressing recombinant human erythropoietin (EPO) in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al. 2019) were grown at 37°C under 5% CO2 and 80% humidity at 170 rpm in BalanCD CHO Growth A (FujiFilm, 91128) cell culture medium supplemented with 4 mM L‐glutamine (Sigma Aldrich, G7513) in a Climo‐Shaker (Kühner). .. Cells were cultured in a working volume of 5 mL of cell culture media in 50mL TPP® TubeSpin BioReactor Tubes (Merck, Z761028‐180EA), with routine passaging at 2 × 105 cells/mL.
Article Title: Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions
Article Snippet: .. CHO‐K1 (ATCC CCL‐61), CHO‐DP12 cell line (ATCC CRL‐12445 clone #1934) which produces an anti‐IL8‐IgG, and CHO‐SK15‐EPO (an in‐house CHO‐K1 (ATCC CCL‐61) derived cell line, expressing recombinant human erythropoietin (EPO) in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al. ) were grown at 37°C under 5% CO 2 and 80% humidity at 170 rpm in BalanCD CHO Growth A (FujiFilm, 91128) cell culture medium supplemented with 4 mM l ‐glutamine (Sigma Aldrich, G7513) in a Climo‐Shaker (Kühner). .. Cells were cultured in a working volume of 5 mL of cell culture media in 50 mL TPP® TubeSpin BioReactor Tubes (Merck, Z761028‐180EA), with routine passaging at 2 × 10 5 cells/mL.
Article Title: Label-free quantitative proteomics analysis of producer and non-producer Chinese Hamsters Ovary (CHO) cells under ER stress conditions
Article Snippet: .. CHO-K1 (non-producer, ATCC CCL-61), CHO DP-12 (a recombinant human anti-IL-8 producer, ATCC CRL-12445 clone #1934) and CHO SK15-EPO (an in-house CHO-K1 derived cell line, expressing recombinant human erythropoietin in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al., 2019) were grown in BalanCD CHO Growth A (FujiFilm, NL) supplemented with 4 mM L-glutamine (Sigma Aldrich, IE) and maintained in a ClimoShaker ISF1-X (Kühner, CH) at 37 ◦C under 5% CO2 and 80% humidity with constant shaking (170 RPM). ..
Plasmid Preparation:Article Title: Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions.
Article Snippet: .. CHO‐K1 (ATCC CCL‐61), CHO‐DP12 cell line (ATCC CRL‐ 12445 clone #1934) which produces an anti‐IL8‐IgG, and CHO‐ SK15‐EPO (an in‐house CHO‐K1 (ATCC CCL‐61) derived cell line, expressing recombinant human erythropoietin (EPO) in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al. 2019) were grown at 37°C under 5% CO2 and 80% humidity at 170 rpm in BalanCD CHO Growth A (FujiFilm, 91128) cell culture medium supplemented with 4 mM L‐glutamine (Sigma Aldrich, G7513) in a Climo‐Shaker (Kühner). .. Cells were cultured in a working volume of 5 mL of cell culture media in 50mL TPP® TubeSpin BioReactor Tubes (Merck, Z761028‐180EA), with routine passaging at 2 × 105 cells/mL.
Article Title: Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions
Article Snippet: .. CHO‐K1 (ATCC CCL‐61), CHO‐DP12 cell line (ATCC CRL‐12445 clone #1934) which produces an anti‐IL8‐IgG, and CHO‐SK15‐EPO (an in‐house CHO‐K1 (ATCC CCL‐61) derived cell line, expressing recombinant human erythropoietin (EPO) in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al. ) were grown at 37°C under 5% CO 2 and 80% humidity at 170 rpm in BalanCD CHO Growth A (FujiFilm, 91128) cell culture medium supplemented with 4 mM l ‐glutamine (Sigma Aldrich, G7513) in a Climo‐Shaker (Kühner). .. Cells were cultured in a working volume of 5 mL of cell culture media in 50 mL TPP® TubeSpin BioReactor Tubes (Merck, Z761028‐180EA), with routine passaging at 2 × 10 5 cells/mL.
Modification:Article Title: Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions.
Article Snippet: .. CHO‐K1 (ATCC CCL‐61), CHO‐DP12 cell line (ATCC CRL‐ 12445 clone #1934) which produces an anti‐IL8‐IgG, and CHO‐ SK15‐EPO (an in‐house CHO‐K1 (ATCC CCL‐61) derived cell line, expressing recombinant human erythropoietin (EPO) in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al. 2019) were grown at 37°C under 5% CO2 and 80% humidity at 170 rpm in BalanCD CHO Growth A (FujiFilm, 91128) cell culture medium supplemented with 4 mM L‐glutamine (Sigma Aldrich, G7513) in a Climo‐Shaker (Kühner). .. Cells were cultured in a working volume of 5 mL of cell culture media in 50mL TPP® TubeSpin BioReactor Tubes (Merck, Z761028‐180EA), with routine passaging at 2 × 105 cells/mL.
Article Title: Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions
Article Snippet: .. CHO‐K1 (ATCC CCL‐61), CHO‐DP12 cell line (ATCC CRL‐12445 clone #1934) which produces an anti‐IL8‐IgG, and CHO‐SK15‐EPO (an in‐house CHO‐K1 (ATCC CCL‐61) derived cell line, expressing recombinant human erythropoietin (EPO) in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al. ) were grown at 37°C under 5% CO 2 and 80% humidity at 170 rpm in BalanCD CHO Growth A (FujiFilm, 91128) cell culture medium supplemented with 4 mM l ‐glutamine (Sigma Aldrich, G7513) in a Climo‐Shaker (Kühner). .. Cells were cultured in a working volume of 5 mL of cell culture media in 50 mL TPP® TubeSpin BioReactor Tubes (Merck, Z761028‐180EA), with routine passaging at 2 × 10 5 cells/mL.
Article Title: Label-free quantitative proteomics analysis of producer and non-producer Chinese Hamsters Ovary (CHO) cells under ER stress conditions
Article Snippet: .. CHO-K1 (non-producer, ATCC CCL-61), CHO DP-12 (a recombinant human anti-IL-8 producer, ATCC CRL-12445 clone #1934) and CHO SK15-EPO (an in-house CHO-K1 derived cell line, expressing recombinant human erythropoietin in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al., 2019) were grown in BalanCD CHO Growth A (FujiFilm, NL) supplemented with 4 mM L-glutamine (Sigma Aldrich, IE) and maintained in a ClimoShaker ISF1-X (Kühner, CH) at 37 ◦C under 5% CO2 and 80% humidity with constant shaking (170 RPM). ..
Selection:Article Title: Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions.
Article Snippet: .. CHO‐K1 (ATCC CCL‐61), CHO‐DP12 cell line (ATCC CRL‐ 12445 clone #1934) which produces an anti‐IL8‐IgG, and CHO‐ SK15‐EPO (an in‐house CHO‐K1 (ATCC CCL‐61) derived cell line, expressing recombinant human erythropoietin (EPO) in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al. 2019) were grown at 37°C under 5% CO2 and 80% humidity at 170 rpm in BalanCD CHO Growth A (FujiFilm, 91128) cell culture medium supplemented with 4 mM L‐glutamine (Sigma Aldrich, G7513) in a Climo‐Shaker (Kühner). .. Cells were cultured in a working volume of 5 mL of cell culture media in 50mL TPP® TubeSpin BioReactor Tubes (Merck, Z761028‐180EA), with routine passaging at 2 × 105 cells/mL.
Article Title: Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions
Article Snippet: .. CHO‐K1 (ATCC CCL‐61), CHO‐DP12 cell line (ATCC CRL‐12445 clone #1934) which produces an anti‐IL8‐IgG, and CHO‐SK15‐EPO (an in‐house CHO‐K1 (ATCC CCL‐61) derived cell line, expressing recombinant human erythropoietin (EPO) in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al. ) were grown at 37°C under 5% CO 2 and 80% humidity at 170 rpm in BalanCD CHO Growth A (FujiFilm, 91128) cell culture medium supplemented with 4 mM l ‐glutamine (Sigma Aldrich, G7513) in a Climo‐Shaker (Kühner). .. Cells were cultured in a working volume of 5 mL of cell culture media in 50 mL TPP® TubeSpin BioReactor Tubes (Merck, Z761028‐180EA), with routine passaging at 2 × 10 5 cells/mL.
Article Title: Label-free quantitative proteomics analysis of producer and non-producer Chinese Hamsters Ovary (CHO) cells under ER stress conditions
Article Snippet: .. CHO-K1 (non-producer, ATCC CCL-61), CHO DP-12 (a recombinant human anti-IL-8 producer, ATCC CRL-12445 clone #1934) and CHO SK15-EPO (an in-house CHO-K1 derived cell line, expressing recombinant human erythropoietin in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al., 2019) were grown in BalanCD CHO Growth A (FujiFilm, NL) supplemented with 4 mM L-glutamine (Sigma Aldrich, IE) and maintained in a ClimoShaker ISF1-X (Kühner, CH) at 37 ◦C under 5% CO2 and 80% humidity with constant shaking (170 RPM). ..
Cell Culture:Article Title: Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions.
Article Snippet: .. CHO‐K1 (ATCC CCL‐61), CHO‐DP12 cell line (ATCC CRL‐ 12445 clone #1934) which produces an anti‐IL8‐IgG, and CHO‐ SK15‐EPO (an in‐house CHO‐K1 (ATCC CCL‐61) derived cell line, expressing recombinant human erythropoietin (EPO) in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al. 2019) were grown at 37°C under 5% CO2 and 80% humidity at 170 rpm in BalanCD CHO Growth A (FujiFilm, 91128) cell culture medium supplemented with 4 mM L‐glutamine (Sigma Aldrich, G7513) in a Climo‐Shaker (Kühner). .. Cells were cultured in a working volume of 5 mL of cell culture media in 50mL TPP® TubeSpin BioReactor Tubes (Merck, Z761028‐180EA), with routine passaging at 2 × 105 cells/mL.
Article Title: Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions
Article Snippet: .. CHO‐K1 (ATCC CCL‐61), CHO‐DP12 cell line (ATCC CRL‐12445 clone #1934) which produces an anti‐IL8‐IgG, and CHO‐SK15‐EPO (an in‐house CHO‐K1 (ATCC CCL‐61) derived cell line, expressing recombinant human erythropoietin (EPO) in a pcDNA3.1 vector (Invitrogen) modified with puromycin resistance as selection system) (Costello et al. ) were grown at 37°C under 5% CO 2 and 80% humidity at 170 rpm in BalanCD CHO Growth A (FujiFilm, 91128) cell culture medium supplemented with 4 mM l ‐glutamine (Sigma Aldrich, G7513) in a Climo‐Shaker (Kühner). .. Cells were cultured in a working volume of 5 mL of cell culture media in 50 mL TPP® TubeSpin BioReactor Tubes (Merck, Z761028‐180EA), with routine passaging at 2 × 10 5 cells/mL.
|
ATCC is a verified supplier 
ATCC manufactures this product 